Introduction

The rapid cooking bean project (RCBP) is a collaborative project of The University of Western Australia (UWA), Alliance Bioversity International and CIAT, and national bean programmes in six partner countries (PCs) in east Africa (UGA, RWA, ETH, BDI, TZA, KEN) linked by the Pan African Bean Research Alliance (PABRA), and funded primarily through the Australian Centre for International Agricultural Research (ACIAR).

Objectives

1. Evaluate yield, adaptation and quality across six partner countries and Alliance Bioversity International and CIAT -Kawanda.

2. Select the best parents among an African common bean panel to be used for crossing so as to maximize future genetic progress for the benefit of all PC’s.

3. Optimize the analysis of genotype x environment interactions (GxE) for these traits which will help to identify the best parents for crossing.

4. Utilize the collected data to evaluate genomic breeding values for important traits.

Trial design

Due to low seed amounts, trial design is partially replicated using DiGGer software at each site with some varieties in single replications and others in two replications. Not all varieties can be sown at every site. Local controls are added following your advice. There is a different design in each PC

Data Collection

Trial location data

Name of trial site (village, district and nearest city)
Altitude of trial site (meters above the sea level (m.a.s.l) measured with an altimeter; or by map position
GPS location (latitude and longitude coordinates) measured with GPS, in degrees and minutes; or by map position
Soil data: Texture, P, N, K, OM, pH, soil composition (% sand, silt, clay).

Management data

Planting date
Method of planting (manual or mechanical)
Type of seed bed (flat or ridged)
Frequency and type of weeding (manual, mechanical, or if herbicide then state the name of the herbicide frequency and rate of application)
Frequency, type, and rate of pesticide application
Timing and amount of irrigation if any
Timing, amount, distribution, placement, and type of fertilizer

Weather data

Daily minimum and maximum temperature (during crop growth period) Daily rainfall data (during crop growth period)

Crop response data

Name of Trait	Abbreviated name	Description of Trait	Describe how measured (method)	Growth stages	Rating scale/units	PC	Comments
Growth habit	GH	A qualitative description of the growth habit of the plant.	Based on visual classification by categories (see appendix 2)	R6,R7	1-5	All
Days to flowering	DF	Number of days from planting to day when 50% of plants in plot have at least one flower	Evaluated visually. See "Stages of development of the common bean plant".	R6	days	All
Halo blight on leaves in field	HBFL	Disease reaction to Pseudomonas on leaves; field	Quantification of symptom development; Flowering (R6) (see appendix 2)	R6	1-9	All
Rust on leaves in field	RUSTFL	Disease reaction to Uromyces phaseoli on leaves; field	Quantification of symptom development; Flowering (R6) (see appendix 2)	R6	1-9	All
Days to physiological maturity	DPM	Number of days from planting to day when the first pod begins to discolor in 50% of the plants.	Evaluated visually. See "Stages of development of the common bean plant".	R9	days	All
Number of plants per effective plot area	PLPLOT	Number of plants per effective plot area	Number of plants in effective plot after discarding 0.5 meter from each end of the plot	Normally at harvest	number	All
Pods per plant	PDPL	Number of pods counted per plant grown in a pot or the field	Average number of pods from 5 to 10 randomly selected plants	R9, Harvest	number	All
Seeds per pod	SDPD	Number of seeds per pod	Average number of seeds/pod in 10 pods, one from each of 5-10 randomly selected plants	R9, Harvest	number	All
Seed production per effective plot	GMPLOT	Weight of seed produced in the effective plot area (g)	Weight (g) of seed harvest (at 13% moisture content) from all plants in the effective plot area	Harvest	g	All	Seed moisture content should be 13%
Yield per plant	YDPL	Average yield per plant in effective plot area	Total yield of effective plot (GMPLOT) divided by number of plants in effective plot area	Harvest	g	All	Calculated from GMPLOT
Yield per hectare	YDHA	Estimation of yield per hectare (kg/ha)	Weight (kg) of seed harvest divided by effective plot area in m² and multiplied by 10.	Harvest	kg/ha	All	Calculated from GMPLOT
Seed weight	100SW	Weight of 100 seeds (g)	100 seeds (at 13% moisture content) are weighed on an analytical scale	Harvest or later	g	All	Seed moisture content should be 13%
Pythium spp. in field	PYTHF	Disease reaction to Pythium spp; field	Quantification of symptom development; Emergence (V1), Flowering (R6); Pod Formation (R7), Pod filling (R8).	V1,R6,R7,R8	1-9	KEN
Bean Common Mosaic Necrosis Virus	BCMNV	Reaction to viral infection	Evaluated as mosaic (M); necrotic (N); asymptomatic (O), local lesions (L) or variable (V). (see appendix 2)	R5-R7	1-5	UGA RWA BDI
Bean Stem Maggot	BSM	Reaction to Ophiomyia spp	Measured on 1 to 9 scale (see appendix 2). Evaluation Third trifoliate leaf (V4).	V4	1-9	ETH UGA
Angular Leaf Spot on leaves in field	ALSF	Disease reaction to P. griseola on leaves; field	Quantification of symptom development; Flowering (R6). (see appendix 2)	R6	1-9	RWA BDI
Angular Leaf Spot on pods in field	ALSFP	Disease reaction to P. griseola on pods; field	Quantification of symptom development; Pod filling (R8).	R8	1-9	RWA BDI
Anthracnose on leaves in field	ANTFL	Disease reaction to C. lindemuthianum on leaves; field	Quantification of symptom development in leaves; Flowering (R6). (see appendix 2)	R6	1-9	KEN
Anthracnose on pods in field	ANTFP	Disease reaction to C. lindemuthianum on pods; field	Quantification of symptom development in pods; Pod Formation (R7), Pod filling (R8). (see appendix 2)	R7,R8	1-9	KEN
Common Bacterial Blight on leaves in field	CBBFL	Disease reaction to Axonopodis on leaves; field	Quantification of symptom development; Flowering (R6). (see appendix 2)	R6	1-9	UGA
Common Bacterial Blight on pods in field	CBBFP	Disease reaction to Axonopodis on pods; field	Quantification of symptom development; Pod Formation (R7), Pod filling (R8). (see appendix 2)	R7,R8	1-9	UGA
Bruchid damage	BRU	Quantification of bruchid damage on seed	Adult emergence from seed as percentage of egg count	Post Harvest	%	ETH UGA
Fusarium solani in field	FUSRRF	Disease reaction to Fusarium solani; field	Quantification of symptom development; Emergence (V1), Flowering (R6); Pod Formation (R7), Pod filling (R8) (see appendix 2)	V1,R6,R7,R8	1-9	KEN
Seed Zinc	ZNSEED	Zinc content in seed	Measured by XRF methods in ppm	Post Harvest	ppm	RWA CIAT-Kw
Seed Iron	FESEED	Iron content in seed	Measured by XRF methods in ppm	Post Harvest	ppm	RWA CIAT-Kw
Cooking time	COOKT	Time taken for 96 to100% of grain to reach soft texture	Measured by Mattson cooker in minutes	Post Harvest	minutes	CIAT-Kw
Low soil fertility			This is often done by setting the trail in poor soils (low P, N or high Al) and observing the root development and plant development and finally the yield parameters.			TZA BDI
Number of effective nodules	NENBB/ NENCB	Quantity of nodules of Rhizobium spp. in the plant	It is expressed as the number of effective nodules (red or pink) per plant (see appendix 2)	R6	1-9	BDI

Rating Scale

Trait #	Name of Trait	Abbreviated name	Rating scale	Scale Description
	Growth habit	GH	1-5	1=Determinate bush
				2=Indeterminate bush habit, erect stems and branches
				3=Indeterminate bush habit with weak main stem and prostrate stem and branches
				4=Indeterminate climber habit with weak, long and twisted stem and branches
				5=Determinate climber
	Angular Leaf Spot on leaves in field	ALSF	1-9	1= No visible disease symptoms
				2= Unspecified intermediate values correspond to intermediate percentages of affected areas
				3= Presence of a few small non-sporulating lesions that cover approximately 2% of the leaf surface area,
				4= Unspecified intermediate values correspond to intermediate percentages of affected areas
				5= Presence of several small lesions with limited sporulation that cover approximately 5% of the leaf surface area
				6= Unspecified intermediate values correspond to intermediate percentages of affected areas
				7= Abundant and generally large sporulating lesions that cover approximately 10% of the leaf surface area. On the foliage, the lesions may coalesce to produce larger infected areas associated with chlorotic tissue. Lesions may also be found on the stem an*
				8= Unspecified intermediate values correspond to intermediate percentages of affected areas
				9= 25% or more of the leaf surface area is covered by large sporulating and often coalescing lesions. Leaf tissues are generally chlorotic resulting in severe and premature defoliation. Abundant sporulating lesions are present on stem and branches
	Angular Leaf Spot on pods in field	ALSFP	1-9	1= No visible disease symptoms
				2= Unspecified intermediate values correspond to intermediate percentages of affected areas
				3= Presence of a few small non-sporulating lesions that cover approximately 2% of the pod surface area
				4= Unspecified intermediate values correspond to intermediate percentages of affected areas
				5= Presence of several small lesions with limited sporulation that cover approximately 5% of the pod surface area
				6= Unspecified intermediate values correspond to intermediate percentages of affected areas
				7= Abundant and generally large sporulating lesions that cover approximately between 10% and 20% of the pod surface area
				8= Unspecified intermediate values correspond to intermediate percentages of affected areas
				9= 50% or more of the pod surface area is covered by large sporulating and often coalescing lesions. Infected pods are often deformed and shrivelled and contain a low number of seeds
	Anthracnose on leaves in field	ANTFL	1-9	1= No visible disease symptoms 2= Unspecified intermediate values correspond to intermediate percentages of affected areas 3= Presence of very few and small lesions mostly on the primary vein of the leaf's lower side that cover approximately 1% of the surface area 4= Unspecified intermediate values correspond to intermediate percentages of affected areas 5= Presence of several small lesions on the petiole or on the primary and secondary veins of the leaf's lower side that cover approximately 5% of the leaf surface area 6= Unspecified intermediate values correspond to intermediate percentages of affected areas 7= Presence of numerous enlarged lesions on the lower side of the leaf. Necrotic lesions can be observed on the upper leaf surface and on the petioles that cover approximately 10% of the leaf surface area 8= Unspecified intermediate values correspond to intermediate percentages of affected areas 9= Severe necrosis on 25% or more of the plant tissue including lesions on the leaf, petioles, stem, branches and even on the growing point which often results in death of much of the plant tissues
	Anthracnose on pods in field	ANTFP	1-9	1= No visible disease symptoms 2= Unspecified intermediate values correspond to intermediate percentages of affected areas 3= Presence of very few and small lesions that cover approximately 1% of the pod surface area 4= Unspecified intermediate values correspond to intermediate percentages of affected areas 5= Presence of several small round lesions (less than 2 mm in diameter), with sporulation that cover approximately 5% of the pod surface area 6= Unspecified intermediate values correspond to intermediate percentages of affected areas 7= Presence of medium-sized (larger than 2 mm in diameter) lesions are evident but also some small and large lesions generally with sporulation and that cover approximately between 10% and 20% of the pod surface area 8= Unspecified intermediate values correspond to intermediate percentages of affected areas 9= Presence of numerous, large, sporulating, sunken cankers that cover approximately 50% or more of the pod surface area and that can result in pod malformation, low seed number, and death of the pod
	Bean Stem Maggot	BSM	1-9	1= Infested plants are as vigorous as uninfested plants. The bean fly apparently causes no considerable damage 2= Unspecified intermediate values correspond to intermediate stages of damage 3= Infested plants with slight growth delay 4= Unspecified intermediate values correspond to intermediate stages of damage 5= Infested plants with considerable growth delay 6= Unspecified intermediate values correspond to intermediate stages of damage 7=Infested plants with severe growth delay 8= Unspecified intermediate values correspond to intermediate stages of damage 9= Infested plants dead or almost dead
	Common Bacterial Blight on leaves in field	CBBFL	1-9	1= No visible disease symptoms 2= Unspecified intermediate values correspond to intermediate percentages of affected areas 3= Presence of a few small lesions that cover approximately 2% of the leaf surface area 4= Unspecified intermediate values correspond to intermediate percentages of affected areas 5= Approximately 5% of the leaf surface area covered by small lesions that are beginning to coalesce and sometimes encircled by yellow halos resulting in minor blight 6= Unspecified intermediate values correspond to intermediate percentages of affected areas 7= Approximately 10% of the leaf surface area covered with medium and large lesions which are usually accompanied by yellow halos and necrosis 8= Unspecified intermediate values correspond to intermediate percentages of affected areas 9= 25% or more of the leaf surface area is covered by large coalescing and g
	Common Bacterial Blight on pods in field	CBBFP	1-9	1= No visible disease symptoms 2= Unspecified intermediate values correspond to intermediate percentages of affected areas 3= Presence of a few small lesions that cover approximately 2% of the pod surface area 4= Unspecified intermediate values correspond to intermediate percentages of affected areas 5= Approximately 5% of the pod surface area covered by small and not coalescing lesions 6= Unspecified intermediate values correspond to intermediate percentages of affected areas 7= Approximately 10% of the pod surface area is covered by large and coalescing lesions and often show bacterial exudate 8= Unspecified intermediate values correspond to intermediate percentages of affected areas 9= 25% or more of the pod surface area is covered by large coalescing lesions that
	Fusarium solani in field	FUSRRF	1-9	1= No visible disease symptoms 2= Unspecified intermediate values correspond to intermediate percentages of damage 3= Light discoloration either without necrotic lesions or with approximately 10% of the hypocotyl and root tissues covered with lesions 4= Unspecified intermediate values correspond to intermediate percentages of damage 5= Approximately 25% of the hypocotyl and root tissues covered with lesions but tissues remain firm with deterioration of the root system. Heavy discoloration symptoms may be evident 6= Unspecified intermediate values correspond to intermediate percentages of damage 7= Approximately 50% of the hypocotyl and root tissues covered with lesions combined with considerable softening, rotting, and reduction of the root system 8= Unspecified intermediate values correspond to intermediate percentages of damage 9= Approximately 75% or more of the hypocotyl and root tissues affected with ad
	Halo blight on leaves in field	HBFL	1-9	1= No visible disease symptoms 2= Unspecified intermediate values correspond to intermediate percentages of affected areas 3= Approximately 2% of the leaf surface area covered with round lesions. Very slight systemic chlorosis may be evident 4= Unspecified intermediate values correspond to intermediate percentages of affected areas 5= Approximately 5% of the leaf surface area covered with round lesions of about 5 mm in diameter. Limited systemic chlorosis may be present on growing points 6= Unspecified intermediate values correspond to intermediate percentages of affected areas 7= Approximately 10% of the leaf tissues affected either by lesions or by the resulting chlorosis. Limited leaf distortion is presented 8= Unspecified intermediate values correspond to intermediate percentages of affected areas 9= 25% or more of the leaf tissues affected by lesions and chlorosis. S
	Rust on leaves in field	RUSTFL	1-9	1= Highly resistant: no visible rust pustule present (immune) 2= Unspecified intermediate values correspond to intermediate percentages of affected areas. 3= Resistant: presence of only a few and generally small pustules on most plants that cover approximately 2% of the foliar area 4= Unspecified intermediate values correspond to intermediate percentages of affected areas. 5= Intermediate: presence of generally small or intermediate pustules on all plants that cover approximately 5% of the foliar area 6= Unspecified intermediate values correspond to intermediate percentages of affected areas. 7= Susceptible: presence of mostly large pustules often surrounded by chlorotic halos that cover approximately 10% of the foliar area 8= Unspecified intermediate values correspond to intermediate percentages of affected areas. 9=Highly susceptible: presence of large and very large pustules with chlorotic halos, that c
	Pythium spp. in field	PYTHF	1-9	1= No visible disease symptoms 2= Unspecified intermediate values correspond to intermediate percentages of damage 3= Light discoloration either without necrotic lesions or with approximately 10% of the hypocotyl and root tissues covered with lesions 4= Unspecified intermediate values correspond to intermediate percentages of damage 5= Approximately 25% of the hypocotyl and root tissues covered with lesions but tissues remain firm with deterioration of the root system. Heavy discoloration symptoms may be evident 6= Unspecified intermediate values correspond to intermediate percentages of damage 7= Approximately 50% of the hypocotyl and root tissues covered with lesions combined with considerable softening, rotting, and reduction of the root system 8= Unspecified intermediate values correspond to intermediate percentages of damage 9= Approximately 75% or more of the hypocotyl and root tissues af
	Bean Common Mosaic Necrosis Virus	BCMNV	1-5	1=M=Mosaic, susceptible 2=N=Necrotic reaction, has I gene 3=O=No symptoms, has recessive resistance 4=L=Local lesions, has protected I gene 5=V=Variable
	Number of effective nodules in bush bean	NENBB	1-9	1=Higher of 80 nodules 2=From 41 to 80 nodules of large size 3=From 41 to 80 nodules of normal or small size 4=From 21 to 40 nodules of large size 5=From 21 to 40 nodules of normal size 6=From 21 to 40 nodules of small size 7=From 10 to 20 nodules of normal size 8=From 10 to 20 nodules of small size 9=Less of 10 nodules
	Number of effective nodules in climbing bean	NENCB	1-9	1=Higher of 240 nodules 2=From 121 to 240 nodules of large size 3=From 121 to 240 nodules of normal or small size 4=From 61 to 120 nodules of large size 5=From 61 to 120 nodules of normal size 6=From 61 to 120 nodules of small size 7=From 30 to 60 nodules of normal size 8=From 30 to 60 nodules of small size 9=Less of 30 nodules

Sample RCBP field trial layout

Field layout

Publication Information

Downloads

RCBP Trial Evaluation Protocol